Enrichment broth used for the isolation of Salmonella species from feces, urine, water, food, and other materials. Gram(+) bacteria are inhibited by Sodium Selenite. The role of Disodium Phosphate is twofold: it maintains a stable pH and reduces the toxicity of selenite, thereby increasing the medium's capacity. The medium allows the growth of Salmonella while inhibiting enterobacteria and enterococci. Inoculate 0.5 – 1g of the sample into 10ml of selenite. Incubate the tubes with loosened caps at 35 – 37°C under aerobic conditions. Check the tubes after 12 – 24 hours of incubation. The presence of microbes is indicated by turbidity or precipitate in the medium. The tubes should be stored for one week before being classified as negative. WARNING for SODIUM BISELENITE: Toxic substance by inhalation and ingestion. Risk of cumulative effects. Causes severe burns. After skin contact, wash immediately with water. If you feel unwell, seek medical advice.